Abstract
Female patients experience substantial neuroprotection after experimental stroke compared with male patients, a finding attributed to the protective effects of gonadal hormones. This study examined the response of male- and female-derived organotypic hippocampal slices to oxidative and excitotoxic injury. Both oxygen and glucose deprivation and N-methyl-D-aspartic acid exposure led to neuronal death; however, female-derived cultures sustained less injury than male-derived cultures. Cell death after oxygen and glucose deprivation was ameliorated in male cultures, but not female cultures, by the addition of 7-nitroindazole, a neuronal nitric oxide synthase inhibitor. These studies have relevance to researchers investigating neuroprotective agents in mixed sex experiments.
Publication types
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Comparative Study
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Animals, Newborn
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Cell Death / drug effects
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Cell Death / physiology
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DNA-Binding Proteins / genetics
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Disease Models, Animal
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Drug Interactions
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Female
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Genotype
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Glucose / deficiency
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High Mobility Group Proteins / genetics
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Hippocampus / pathology*
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Hypoxia
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Indazoles / therapeutic use
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Male
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N-Methylaspartate / toxicity
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Neurons / drug effects
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Neurons / pathology*
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Neuroprotective Agents / therapeutic use
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Nitric Oxide / metabolism
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Organ Culture Techniques
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RNA, Messenger / biosynthesis
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Rats
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Reverse Transcriptase Polymerase Chain Reaction / methods
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SOXB1 Transcription Factors
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Sex Characteristics*
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Stroke / etiology
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Stroke / pathology*
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Stroke / prevention & control
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Superoxide Dismutase / metabolism
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Time Factors
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Transcription Factors / genetics
Substances
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DNA-Binding Proteins
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High Mobility Group Proteins
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Indazoles
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Neuroprotective Agents
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RNA, Messenger
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SOXB1 Transcription Factors
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Transcription Factors
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Nitric Oxide
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N-Methylaspartate
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Superoxide Dismutase
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Glucose
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7-nitroindazole