Fate of the transferrin receptor during maturation of sheep reticulocytes in vitro: Selective externalization of the receptor

doi:10.1016/0092-8674(83)90040-5

Cell

Volume 33, Issue 3, July 1983, Pages 967-978

https://doi.org/10.1016/0092-8674(83)90040-5 Get rights and content

Abstract

The fate of the transferrin receptor during in vitro maturation of sheep reticulocytes has been followed using FITC- and ¹²⁵l-labeled anti-transferrin-receptor antibodies. Vesicles containing peptides that comigrate with the transferrin receptor on polyacrylamide gels are released during incubation of sheep reticulocytes, tagged with anti-transferrin-receptor anti-bodies. Vesicle formation does not require the presence of the anti-transferrin-receptor antibodies. Using ¹²⁵l-surface-labeled reticulocytes, it can be shown that the ¹²⁵l-labeled material which is released is retained by an immunoaffinity column of the anti-transferrin-receptor antibody. Using reticulocytes tagged with ¹²⁵l-labeled anti-transferrin-receptor antibodies to follow the formation of vesicles, it can be shown that at 0°C or in phosphate-buffered saline the rate of vesicle release is less than that at 37°C in culture medium. There is selective externalization of the antibody-receptor complex since few other membrane proteins are found in the externalized vesicles. The anti-transferrin-receptor antibodies cause redistribution of the receptor into patches that do not appear to be required for vesicle formation.

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Cell

ArticleFate of the transferrin receptor during maturation of sheep reticulocytes in vitro: Selective externalization of the receptor

Abstract

J. Biol. Chem.

Cell

Cell

Arch. Biochem. Biophys.

Immunochemistry

J. Immunol. Immunopathol.

Biochim. Biophys. Acta

J. Biol. Chem.

Int. Rev. Cytol.

J. Biol. Chem.

Blood

Biochim. Biophys. Acta

J. Biol. Chem.

Adv. Immunol.

Anal. Biochem.

Biochim. Biophys. Acta

Release of diacylglycerol-enriched vesicles from erythrocytes with increased intracellular Ca2+

Nature

Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides

Nature

Changes in amino acid transport during red cell maturation

Membrane Biochem.

Electrogenic sodium dependent glycine transport in sheep reticulocytes

Can. J. Biochem.

Studies of the transferrin receptor on both human reticulocytes and nucleated human cells in culture

J. Clin. Invest.

Coated pits, coated vesicles, and receptor-mediated endocytosis

Nature

Identification of transferrin receptors on the surface of human cultured cells

Article
Fate of the transferrin receptor during maturation of sheep reticulocytes in vitro: Selective externalization of the receptor

Release of diacylglycerol-enriched vesicles from erythrocytes with increased intracellular Ca²⁺