RT Journal Article
SR Electronic
T1 Atherosclerosis-related biomarker PABPC1 predicts pan-cancer events
JF Stroke and Vascular Neurology
JO Stroke Vasc Neurol
FD BMJ Publishing Group Ltd
SP 108
OP 125
DO 10.1136/svn-2022-002246
VO 9
IS 2
A1 Lin, Miao
A1 Hu, Liubing
A1 Shen, Si
A1 Liu, Jiyue
A1 Liu, Yanyan
A1 Xu, Yixian
A1 Chen, Honglin
A1 Sugimoto, Kazuo
A1 Li, Jianshuang
A1 Kamitsukasa, Ikuo
A1 Hiwasa, Takaki
A1 Wang, Hao
A1 Xu, Anding
YR 2024
UL http://svn.bmj.com/content/9/2/108.abstract
AB Background Atherosclerosis (AS) and tumours are the leading causes of death worldwide and share common risk factors, detection methods and molecular markers. Therefore, searching for serum markers shared by AS and tumours is beneficial to the early diagnosis of patients.Methods The sera of 23 patients with AS-related transient ischaemic attack were screened by serological identification of antigens through recombinant cDNA expression cloning (SEREX), and cDNA clones were identified. Pathway function enrichment analysis was performed on cDNA clones to identify their biological pathways and determine whether they were related to AS or tumours. Subsequently, gene–gene and protein–protein interactions were performed and AS-associated markers would be discovered. The expression of AS biomarkers in human normal organs and pan-cancer tumour tissues were explored. Then, immune infiltration level and tumour mutation burden of various immune cells were evaluated. Survival curves analysis could show the expression of AS markers in pan-cancer.Results AS-related sera were screened by SEREX, and 83 cDNA clones with high homology were obtained. Through functional enrichment analysis, it was found that their functions were closely related to AS and tumour functions. After multiple biological information interaction screening and the external cohort validating, poly(A) binding protein cytoplasmic 1 (PABPC1) was found to be a potential AS biomarker. To assess whether PABPC1 was related to pan-cancer, its expression in different tumour pathological stages and ages was screened. Since AS-associated proteins were closely related to cancer immune infiltration, we investigated and found that PABPC1 had the same role in pan-cancer. Finally, analysis of Kaplan-Meier survival curves revealed that high PABPC1 expression in pan-cancer was associated with high risk of death.Conclusions Through the findings of SEREX and bioinformatics pan-cancer analysis, we concluded that PABPC1 might serve as a potential biomarker for the prediction and diagnosis of AS and pan-cancer.All data relevant to the study are included in the article or uploaded as online supplemental information. All data generated or analyzed during this study are included in this published article and its supplemental information files, and are available from the corresponding author on reasonable request.