RT Journal Article
SR Electronic
T1 Long-term modification of gut microbiota by broad-spectrum antibiotics improves stroke outcome in rats
JF Stroke and Vascular Neurology
JO Stroke Vasc Neurol
FD BMJ Publishing Group Ltd
SP svn-2021-001231
DO 10.1136/svn-2021-001231
A1 Chang Liu
A1 Xi Cheng
A1 Shanshan Zhong
A1 Zhouyang Liu
A1 Fangxi Liu
A1 Xinyu Lin
A1 Yinan Zhao
A1 Meiting Guan
A1 Ting Xiao
A1 Jukka Jolkkonen
A1 Ying Wang
A1 Chuansheng Zhao
YR 2022
UL http://svn.bmj.com/content/early/2022/05/15/svn-2021-001231.abstract
AB Background The brain-gut axis is a major regulator of the central nervous system. We investigated the effects of treatment with broad-spectrum antibiotics on gut and brain inflammation, infarct size and long-term behavioral outcome after cerebral ischemia in rats.Methods Rats were treated with broad-spectrum antibiotics (ampicillin, vancomycin, ciprofloxacin, meropenem and metronidazole) for 4 weeks before the endothelin-1 induced ischemia. Treatment continued for 2 weeks until the end of behavioral testing, which included tapered ledged beam-walking, adhesive label test and cylinder test. Gut microbiome, short-chain fatty acids and cytokine levels were measured together with an assessment of infarct size, neuroinflammation and neurogenesis.Results The results revealed that the antibiotics exerted a clear impact on the gut microbiota. This was associated with a decrease in systemic and brain cytokine levels, infarct size and apoptosis in the perilesional cortex and improved behavioral outcome.Conclusion Our results highlighted the significant relationship between intestinal microbiota and beneficial neuro-recovery after ischemic stroke.Data are available upon reasonable request. The data contains the original images as well as the original files, the raw data are available from the authors CL and CZ (leo_liuchang@163.com and cszhao@cmu.edu.cn). Permission to reuse the data need to be granted with the permission of the journal and the corresponding author.