PT  - JOURNAL ARTICLE
AU  - Ming Zou
AU  - Yan Feng
AU  - Yuwhen Xiu
AU  - Yan Li
AU  - Ying Zhang
AU  - Junwan Fan
AU  - Haowen Li
AU  - Jingli Cao
AU  - Wenyan He
AU  - Wei-Na Jin
TI  - Pertussis toxin–induced inflammatory response exacerbates intracerebral haemorrhage and ischaemic stroke in mice
AID  - 10.1136/svn-2021-000987
DP  - 2022 Feb 01
TA  - Stroke and Vascular Neurology
PG  - 29--37
VI  - 7
IP  - 1
4099  - http://svn.bmj.com/content/7/1/29.short
4100  - http://svn.bmj.com/content/7/1/29.full
SO  - Stroke Vasc Neurol2022 Feb 01; 7
AB  - Background Stroke is a devastating disease, including intracerebral haemorrhage (ICH) and ischaemic stroke. Emerging evidences indicate that systemic inflammatory cascades after stroke contribute to brain damage. However, the direct effects and features of systemic inflammation on brain injury, especially comparing between ischaemic and haemorrhagic stroke, are still obscure.Methods Pertussis toxin (PT) was used to build a pro-inflammatory milieu after ICH and ischaemic stroke in mouse model. The neurodeficits, stroke lesion, immune response and blood–brain barrier (BBB) destruction were assessed.Results In ICH mouse model, PT-induced systemic inflammation exacerbated neurological deficits, and enlarged haemorrhage lesion and perihaematomal oedema. We also found promoted leucocyte infiltration and inflammatory cytokine release into the brain after PT treatment. Moreover, the integrity of the BBB was further disrupted after receiving PT. Furthermore, we demonstrated that PT enhanced brain inflammation and aggravated stroke severity in middle cerebral artery occlusion mouse model.Conclusions Our results suggest that PT increases inflammatory response that exacerbates brain injury after ICH or ischaemic stroke in mouse model.All data relevant to the study are included in the article or uploaded as online supplemental information. All data generated or analysed during this study are included in this published article and its supplemental information files, and are available from the corresponding author on reasonable request.